Systematic surveillance detects multiple silent introductions and household transmission of methicillin-resistant Staphylococcus aureus USA300 in the east of England.


Toleman, MS; Reuter, S; Coll, F; Harrison, EM; Blane, B; Brown, NM; Török, ME; Parkhill, J; Peacock, SJ; (2016) Systematic surveillance detects multiple silent introductions and household transmission of methicillin-resistant Staphylococcus aureus USA300 in the east of England. The Journal of infectious diseases. ISSN 0022-1899 DOI: 10.1093/infdis/jiw166

[img] Text - Published Version
License:

Download (456kB)

Abstract

 The spread of USA300 methicillin-resistant Staphylococcus aureus (MRSA) across the United States (US) resulted in an epidemic of infections. In Europe, only sporadic cases or small clusters of USA300 infections are described and its prevalence in England is unknown. We conducted prospective surveillance for USA300 in the east of England.  We undertook a 12-month prospective observational cohort study of all individuals with MRSA isolated from community and hospital samples submitted to a microbiology laboratory. At least one MRSA isolate from each individual was whole-genome sequenced. USA300 was identified based on sequence analysis, and phylogenetic comparisons were made between these and USA300 genomes from the US.  Between April 2012-April 2013, we sequenced 2,283 MRSA isolates (carriage screens and clinical samples) from 1,465 individuals. USA300 was isolated from 24 (1.6%) cases. Ten cases (42%) had skin and soft tissue infection and two cases had invasive disease. Phylogenetic analyses identified multiple introductions and household transmission of USA300.  Use of a diagnostic laboratory as a sentinel for surveillance has identified repeated introductions of USA300 into the east of England in 2012-2013, with evidence for limited transmission. Our results show how systematic surveillance could provide an early-warning of strain emergence and dissemination.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 27122590
URI: http://researchonline.lshtm.ac.uk/id/eprint/2548516

Statistics


Download activity - last 12 months
Downloads since deposit
13Downloads
37Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item