An evaluation of the Luminex xTAG Gastrointestinal Pathogen Panel assay for the detection of multiple diarrheal pathogens in fecal samples in Vietnam.


Duong, VT; Vinh Phat, V; Thanh Tuyen, H; Dung, TT; Trung, PD; Minh, PV; Thi Phuong Tu, L; Campbell, JI; Phuc, HL; Ha, TT; Ngoc, NM; Huong, NT; Thi Thanh Tam, P; Huong, DT; Xang, NV; Dong, N; Phuong, LT; Hung, NV; Phu, BD; Phuc, TM; Thwaites, GE; Vi, LL; Rabaa, MA; Thompson, CN; Baker, S; (2016) An evaluation of the Luminex xTAG Gastrointestinal Pathogen Panel assay for the detection of multiple diarrheal pathogens in fecal samples in Vietnam. Journal of clinical microbiology. ISSN 0095-1137 DOI: https://doi.org/10.1128/JCM.03321-15

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Abstract

Diarrheal disease is a complex syndrome that remains a leading cause of childhood morbidity and mortality globally. Diagnosing enteric pathogens in a timely and precise manner is important for treatment decisions and informing public health policy; accurate diagnosis is a major challenge in industrializing countries. Multiplex molecular diagnostic techniques may represent a significant improvement over classical approaches. We evaluated the Luminex xTAG Gastrointestinal Pathogen Panel (GPP) assay for the detection of common enteric bacterial and viral pathogens in Vietnam. Microbiological culture and real-time PCR were used as gold standards. Tests were performed on 479 stool samples collected from people admitted to hospital for diarrheal disease throughout Vietnam. Sensitivity and specificity were calculated for the xTAG GPP for the seven principal diarrheal etiologies. The sensitivity and specificity for the xTAG GPP were >88% for Shigella spp., Campylobacter spp., rotavirus, norovirus GI/GII and adenovirus compared to microbiological culture and/or real-time PCR. However, the specificity was low (∼60%) for Salmonella spp. Additionally, a number of important pathogens that are not identified in routine hospital procedures in this setting such as Cryptosporidium spp. and Clostridium difficile were detected. The use of the Luminex xTAG GPP for the detection of enteric pathogens in settings like Vietnam would dramatically improve the diagnostic accuracy and capacity of hospital laboratories, allowing for timely and appropriate therapy decisions as well as a wider understanding of the epidemiology of pathogens associated with severe diarrheal disease in low resource settings.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 26865681
Web of Science ID: 373337100034
URI: http://researchonline.lshtm.ac.uk/id/eprint/2530914

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