Utility of cox1 phylogenetics to differentiate between locally acquired and imported Plasmodium knowlesi infections in Singapore.


Loh, JP; Gao, QH; Lee, VJ; Tetteh, K; Drakeley, C; (2016) Utility of cox1 phylogenetics to differentiate between locally acquired and imported Plasmodium knowlesi infections in Singapore. Singapore medical journal, 57 (12). pp. 686-689. ISSN 0037-5675 DOI: https://doi.org/10.11622/smedj.2016016

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Abstract

: Although there have been several phylogenetic studies on Plasmodium knowlesi (P. knowlesi), only cytochrome c oxidase subunit 1 (COX1) gene analysis has shown some geographical differentiation between the isolates of different countries.<br/> : Phylogenetic analysis of locally acquired P. knowlesi infections, based on circumsporozoite, small subunit ribosomal ribonucleic acid (SSU rRNA), merozoite surface protein 1 and COX1 gene targets, was performed. The results were compared with the published sequences of regional isolates from Malaysia and Thailand.<br/> : Phylogenetic analysis of the circumsporozoite, SSU rRNA and merozoite surface protein 1 gene sequences for regional P. knowlesi isolates showed no obvious differentiation that could be attributed to their geographical origin. However, COX1 gene analysis showed that it was possible to differentiate between Singapore-acquired P. knowlesi infections and P. knowlesi infections from Peninsular Malaysia and Sarawak, Borneo, Malaysia.<br/> : The ability to differentiate between locally acquired P. knowlesi infections and imported P. knowlesi infections has important utility for the monitoring of P. knowlesi malaria control programmes in Singapore.<br/>

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Research Centre: Malaria Centre
PubMed ID: 26805667
Web of Science ID: 391298400007
URI: http://researchonline.lshtm.ac.uk/id/eprint/2528483

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