A Small RNA Encoded in the Rv2660c Locus of Mycobacterium tuberculosis Is Induced during Starvation and Infection


Houghton, J; Cortes, T; Schubert, O; Rose, G; Rodgers, A; Croix, MDS; Aebersold, R; Young, DB; Arnvig, KB; (2013) A Small RNA Encoded in the Rv2660c Locus of Mycobacterium tuberculosis Is Induced during Starvation and Infection. PloS one, 8 (12). e80047. ISSN 1932-6203 DOI: https://doi.org/10.1371/journal.pone.0080047

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Abstract

Enhanced transcription of the Rv2660c locus in response to starvation of Mycobacterium tuberculosis H37Rv encouraged addition of the predicted Rv2660c protein to an improved vaccine formulation. Using strand-specific RNA sequencing, we show that the up-regulated transcript is in fact a small RNA encoded on the opposite strand to the annotated Rv2660c. The transcript originates within a prophage and is expressed only in strains that carry PhiRv2. The small RNA contains both host and phage sequences and provides a useful biomarker to monitor bacterial starvation during infection and/or non-replicating persistence. Using different approaches we do not find any evidence of Rv2660c at the level of mRNA or protein. Further efforts to understand the mechanism by which Rv2660c improves efficacy of the H56 vaccine are likely to provide insights into the pathology and immunology of tuberculosis.

Item Type: Article
Keywords: Bacterial Proteins, genetics, Cell Line, Humans, Mycobacterium tuberculosis, genetics, immunology, virology, RNA, Messenger, genetics, Reverse Transcriptase Polymerase Chain Reaction, Tuberculosis, immunology, Virion, genetics
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 24348997
Web of Science ID: 328731800002
URI: http://researchonline.lshtm.ac.uk/id/eprint/2373848

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