IL-18-induced expression of high affinity IL-2R on murine NK cells is essential for NK-cell IFN-γ production during murine Plasmodium yoelii infection.


Stegmann, KA; De Souza, JB; Riley, EM; (2015) IL-18-induced expression of high affinity IL-2R on murine NK cells is essential for NK-cell IFN-γ production during murine Plasmodium yoelii infection. European journal of immunology, 45 (12). pp. 3431-40. ISSN 0014-2980 DOI: 10.1002/eji.201546018

[img] Text - Accepted Version
License:

Download (446kB)
[img]
Preview
Text - Accepted Version
License:

Download (2MB) | Preview
[img]
Preview
Text - Accepted Version
License:

Download (1MB) | Preview
[img]
Preview
Text - Accepted Version
License:

Download (1MB) | Preview
[img]
Preview
Text - Accepted Version
License:

Download (1MB) | Preview
[img]
Preview
Text - Accepted Version
License:

Download (979kB) | Preview

Abstract

: Early production of pro-inflammatory cytokines, including IFN-γ, is essential for control of blood-stage malaria infections. We have shown that IFN-γ production can be induced among human natural killer (NK) cells by coculture with Plasmodium falciparum infected erythrocytes, but the importance of this response is unclear. To further explore the role of NK cells during malaria infection, we have characterized the NK-cell response of C57BL/6 mice during lethal (PyYM) or nonlethal (Py17XNL) P. yoelii infection. Ex vivo flow cytometry revealed that NK cells are activated within 24 h of Py17XNL blood-stage infection, expressing CD25 and producing IFN-γ; this response was blunted and delayed during PyYM infection. CD25 expression and IFN-γ production were highly correlated, suggesting a causal relationship between the two responses. Subsequent in vitro experiments revealed that IL-18 signaling is essential for induction of CD25 and synergizes with IL-12 to enhance CD25 expression on splenic NK cells. In accordance with this, Py17XNL-infected erythrocytes induced NK-cell CD25 expression and IFN-γ production in a manner that is completely IL-18- and partially IL-12-dependent, and IFN-γ production is enhanced by IL-2. These data suggest that IL-2 signaling via CD25 amplifies IL-18- and IL-12-mediated NK-cell activation during malaria infection.<br/>

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Research Centre: Malaria Centre
PubMed ID: 26420375
Web of Science ID: 366402000021
URI: http://researchonline.lshtm.ac.uk/id/eprint/2319282

Statistics


Download activity - last 12 months
Downloads since deposit
280Downloads
223Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item