Analysis of Antibody Responses to Mycobacterium leprae Phenolic Glycolipid-I, Lipoarabinomannan and Recombinant Proteins to Define Disease-Subtype Specific Antigenic Profiles in Leprosy.


Spencer, JS; Kim, HJ; Wheat, WH; Chatterjee, D; Balagon, MV; Cellona, RV; Tan, EV; Gelber, R; Saunderson, P; Duthie, MS; Reece, ST; Burman, W; Belknap, R; Mac Kenzie, WR; Geluk, A; Oskam, L; Dockrell, HM; Brennan, PJ; on behalf of the IDEAL Consortium, ; (2010) Analysis of Antibody Responses to Mycobacterium leprae Phenolic Glycolipid-I, Lipoarabinomannan and Recombinant Proteins to Define Disease-Subtype Specific Antigenic Profiles in Leprosy. Clinical and vaccine immunology . ISSN 1556-6811 DOI: 10.1128/CVI.00472-10

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Abstract

A simple serodiagnostic test based on the Mycobacterium leprae-specific phenolic glycolipid, PGL-I, for individuals with leprosy is nearly universally positive in leprosy patients with high bacillary loads, but cannot be used as a stand-alone diagnostic test for the entire spectrum of the disease process. For patients with early infection with no detectable acid fast bacilli in lesions or with low or no antibody titer to PGL-I as in those at the tuberculoid end of the disease spectrum, this diagnostic approach has limited usefulness. To identify additional M. leprae antigens that might enhance the serological detection of these individuals, we have examined the reactivity patterns of patient sera to PGL-I, lipoarabinomannan (LAM) and six recombinant M. leprae proteins (ML1877, ML0841, ML2028, ML2038, ML0380 and ML0050) by Western blot and ELISA. Overall, the responses to ML2028 (Ag85B) and ML2038 (bacterioferritin) were consistently high in both multibacillary and paucibacillary groups and weak or absent in endemic controls, while responses to other antigens showed considerable variability, from strongly positive to completely negative. This analysis has given a clearer understanding of some of the differences in the antibody responses, between individuals at opposite ends of the disease spectrum, as well as illustrating the heterogeneity of antibody responses towards protein, carbohydrate, and glycolipid antigens within a clinical group. Correlating these response patterns with a particular disease state would allow for a more critical assessment of the form of disease within the leprosy spectrum and could lead to better patient management.

Item Type: Article
Faculty and Department: Academic Services & Administration > Academic Administration
PubMed ID: 21177913
Web of Science ID: 286653900011
URI: http://researchonline.lshtm.ac.uk/id/eprint/1854

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