Evidence for cytoskeletal changes secondary to plasma membrane functional alterations in the in vitro cell response to Clostridium perfringens epsilon-toxin


Donelli, G; Fiorentini, C; Matarrese, P; Falzano, L; Cardines, R; Mastrantonio, P; Payne, DW; Titball, RW; (2003) Evidence for cytoskeletal changes secondary to plasma membrane functional alterations in the in vitro cell response to Clostridium perfringens epsilon-toxin. Comparative immunology, microbiology and infectious diseases, 26 (3). pp. 145-156. ISSN 0147-9571 DOI: 10.1016/S0147-9571(02)00052-8

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Abstract

To investigate the mode of action of Clostridium perfringens epsilon-toxin, MDCK cells were treated with purified toxin and incubated at 37 degreesC for up to 24 h. Exposure to epsilon- toxin caused a time-dependent decrease in cell-cell and cell- substrate interactions. After 30 min of treatment retraction of the cell body and the emission of filopodia were detectable in a number of cells. Longer exposure resulted in cell rounding and cell blebbing which reached a maximum after 5 h of toxin treatment. A parallel modification in the cytoskeleton was also detected. Actin marginalization and the entanglement of microtubules and intermediate filaments were observed by fluorescence microscopy after 30 min of toxin exposure. Functional alterations of the plasma membrane of MDCK cells were assessed by flow cytometry. After 10 or 30 min of intoxication an increase in cell volume was detected, indicating an alteration in plasma membrane permeability. These findings provide evidence for cytoskeletal changes and plasma membrane functional alterations in the in vitro cell response to C. perfringens epsilon-toxin. (C) 2002 Elsevier Science Ltd. All rights reserved.

Item Type: Article
Keywords: Clostridium perfringens, epsilon-toxin, cytoskeleton, membrane, alteration, vascular permeability, Mdck cells, bacteroides-fragilis, escherichia-coli, goats, gene, enterotoxin, binding, Animal, Antibodies, Monoclonal, immunology, Bacterial Toxins, immunology, toxicity, Cell Count, Cell Line, Cell Membrane, drug effects, pathology, physiology, Cell Membrane Permeability, drug effects, Clostridium perfringens, chemistry, Cytoskeleton, drug effects, pathology, Flow Cytometry, veterinary, Kinetics, Membrane Potentials, drug effects, Microscopy, Fluorescence, veterinary
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 12581745
Web of Science ID: 181846500001
URI: http://researchonline.lshtm.ac.uk/id/eprint/17739

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