A mutant of Burkholderia pseudomallei, auxotrophic in the branched chain amino acid biosynthetic pathway, is attenuated and protective in a murine model of melioidosis


Atkins, T; Prior, RG; Mack, K; Russell, P; Nelson, M; Oyston, PCF; Dougan, G; Titball, RW; (2002) A mutant of Burkholderia pseudomallei, auxotrophic in the branched chain amino acid biosynthetic pathway, is attenuated and protective in a murine model of melioidosis. Infection and immunity, 70 (9). pp. 5290-4. ISSN 0019-9567 DOI: 10.1128/iai.70.9.5290-5294.2002

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Abstract

Using a transposon mutagenesis approach, we have identified a mutant of Burkholderia pseudomallei that is auxotrophic for branched chain amino acids. The transposon was shown to have interrupted the ilvI gene encoding the large subunit of the acetolactate synthase enzyme. Compared to the wild type, this mutant was significantly attenuated in a murine model of disease. Mice inoculated intraperitoneally with the auxotrophic mutant, 35 days prior to challenge, were protected against a challenge dose of 6,000 median lethal doses of wild-type B. pseudomallei.

Item Type: Article
Keywords: Dependent salmonella-typhimurium, mycobacterium-bovis bcg, aromatic compound, aroa mutants, balb/c mice, tuberculosis, persistence, leucine, lipopolysaccharide, identification, Amino Acids, Branched-Chain, biosynthesis, Animal, Burkholderia pseudomallei, genetics, immunology, metabolism, pathogenicity, Female, Genotype, Melioidosis, etiology, immunology, prevention & control, Mice, Mice, Inbred BALB C, Mutation, Phenotype, Support, Non-U.S. Gov't
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 12183585
Web of Science ID: 177491100070
URI: http://researchonline.lshtm.ac.uk/id/eprint/17733

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