The ITS-2 of the nuclear rDNA as a molecular marker for populations, species, and phylogenetic relationships in Triatominae (Hemiptera : Reduviidae), vectors of Chagas disease


Marcilla, A; Bargues, MD; Ramsey, JM; Magallon-Gastelum, E; Salazar-Schettino, PM; Abad-Franch, F; Dujardin, JP; Schofield, CJ; Mas-Coma, S; (2001) The ITS-2 of the nuclear rDNA as a molecular marker for populations, species, and phylogenetic relationships in Triatominae (Hemiptera : Reduviidae), vectors of Chagas disease. Molecular phylogenetics and evolution, 18 (1). pp. 136-142. ISSN 1055-7903 DOI: https://doi.org/10.1006/mpev.2000.0864

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Abstract

The nucleotide sequences of the rDNA second internal transcribed spacer (ITS-S) of 31 populations of 12 and 3 species of the two main Triatominae tribes Triatomini and Rhodniini, including the most important Chagas disease vectors, were obtained, Sequence comparisons and parsimony, distance, and maximum-likelihood analyses indicate that ITS-S is a useful marker for resolving supraspecific, specific, subspecific, and even sometimes population-level relationships in Triatominae. Results were markedly different between species of Triatomini and Rhodniini, suggesting polyphyly. Phylogenetic trees support an old divergence between South American and North-Central American Triatomini and query the validity of some genera (Dipetalogaster, Psammolestes). The very low sequence variation between species of the phyllosoma complex suggests that subspecific ranking would be more appropriate. Triatoma dimidiata proves to be a clearly differentiated species, with several populations evidencing a clinal variation along a north-south axis and a population from Yucatan showing differences consistent with specific status. (C) 2001 Academic Press.

Item Type: Article
Keywords: ribosomal DNA, ITS-2, Triatominae, Hemiptera, nucleotide, sequence, phylogenetic analyses, Sequence variation, mitochondrial-dna, reconstruction, substitutions, limits, Animal, Cell Nucleus, metabolism, Chagas Disease, etiology, DNA, Intergenic, DNA, Ribosomal, genetics, Genetic Markers, Likelihood Functions, Phylogeny, Sequence Analysis, DNA, Support, Non-U.S. Gov't, Triatominae, classification, genetics, Variation (Genetics)
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 11161750
Web of Science ID: 166559300015
URI: http://researchonline.lshtm.ac.uk/id/eprint/17712

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