Beta2-microglobulin-dependent bacterial clearance and survival during murine Klebsiella pneumoniae bacteremia


Cogen, AL; Moore, TA; (2009) Beta2-microglobulin-dependent bacterial clearance and survival during murine Klebsiella pneumoniae bacteremia. Infection and immunity, 77 (1). pp. 360-6. ISSN 0019-9567 DOI: https://doi.org/10.1128/IAI.00909-08

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Abstract

Klebsiella pneumoniae is a leading cause of both community-acquired and nosocomial gram-negative bacterial pneumonia. A significant clinical complication of Klebsiella pulmonary infections is peripheral blood dissemination, resulting in a systemic infection concurrent with the localized pulmonary infection. We report here on the critical importance of beta(2)-microglobulin expression during murine K. pneumoniae bacteremia. Beta(2)-microglobulin knockout mice displayed significantly increased mortality upon intravenous inoculation that correlated with increased bacterial burden in the blood, liver, and spleen. As beta(2)-microglobulin knockout mice lack both CD8(+) T cells and invariant NK T cells, mouse models specifically deficient in either cell population were examined to see if this would account for the increased mortality noted in beta(2)-microglobulin knockout mice. Surprisingly, neither CD8 T-cell-deficient (TAP-1 knockout; in vivo anti-CD8 antibody treatment) nor invariant NK (iNK) T-cell-deficient (CD1d knockout, J alpha281 knockout) mice were more susceptible to K. pneumoniae bacteremia. Combined, these studies clearly indicate the importance of a beta(2)-microglobulin-dependent but CD8 T-cell- and iNK T-cell-independent mechanism critical for survival during K. pneumoniae bacteremia.

Item Type: Article
Keywords: Animals, Bacteremia/*immunology/*microbiology, Blood/microbiology, CD8-Positive T-Lymphocytes/immunology, Colony Count, Microbial, Killer Cells, Natural/immunology, Klebsiella pneumoniae/*immunology, Liver/microbiology, Mice, Mice, Inbred C57BL, Mice, Knockout, Spleen/microbiology, Survival Analysis, beta 2-Microglobulin/deficiency/*immunology, Animals, Bacteremia, immunology, microbiology, Blood, microbiology, CD8-Positive T-Lymphocytes, immunology, Colony Count, Microbial, Killer Cells, Natural, immunology, Klebsiella pneumoniae, immunology, Liver, microbiology, Mice, Mice, Inbred C57BL, Mice, Knockout, Spleen, microbiology, Survival Analysis, beta 2-Microglobulin, deficiency, immunology
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
PubMed ID: 18981251
Web of Science ID: 262776100036
URI: http://researchonline.lshtm.ac.uk/id/eprint/1762

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