The failure of different strains of Yersinia pestis to produce lipopolysaccharide O-antigen under different growth conditions is due to mutations in the O-antigen gene cluster


Prior, JL; Parkhill, J; Hitchen, PG; Mungall, KL; Stevens, K; Morris, HR; Reason, AJ; Oyston, PCF; Dell, A; Wren, BW; Titball, RW; (2001) The failure of different strains of Yersinia pestis to produce lipopolysaccharide O-antigen under different growth conditions is due to mutations in the O-antigen gene cluster. FEMS microbiology letters, 197 (2). pp. 229-33. ISSN 0378-1097

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Abstract

The lipopolysaccharide (LPS) from eight strains of Yersinia pestis which had been cultured at 28 degrees C appeared to be devoid of an O-antigen when analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. LPS isolated from three of these strains which had been cultured at 37 degrees C also appeared to be devoid of an O-antigen. When the LPS from Y. pestis strain CO92 was purified and analysed by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry, the observed signals were in the mass range predicted for molecules containing lipid A plus the core oligosaccharide but lacking an O-antigen. The nucleotide sequence of Y. pestis strain CO92 revealed the presence of a putative O-antigen gene cluster. However, frame-shift mutations in the ddhB, gmd, fcl and ushA genes are likely to prevent expression of the O-antigen thus explaining the loss of phenotype.

Item Type: Article
Keywords: lipopolysaccharide, O-antigen, Yersinia pestis, Y-pseudotuberculosis, side-chain, virulence, plague, temperature, infection, o-3, rfb, Comparative Study, Genome, Bacterial, Multigene Family, genetics, Mutation, O Antigens, genetics, Spectrum Analysis, Mass, Support, Non-U.S. Gov't, Temperature, Yersinia pestis, chemistry, genetics, growth & development
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 11313139
Web of Science ID: 168396300015
URI: http://researchonline.lshtm.ac.uk/id/eprint/17392

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