Rotavirus G and P genotypes in rural Ghana


Asmah, RH; Green, J; Armah, GE; Gillimore, CI; Gray, JJ; Iturriza-Gomara, M; Anto, F; Oduro, A; Binka, FN; Brown, DWG; Cutts, F; (2001) Rotavirus G and P genotypes in rural Ghana. Journal of clinical microbiology, 39 (5). pp. 1981-1984. ISSN 0095-1137 DOI: 10.1128/JCM.39.5.1981-1984.2001

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Abstract

An epidemiological study of rotavirus infection was conducted on specimens collected from patients with gastroenteritis and domiciled in the rural Upper Eastern Region of Ghana during 1998, Fifty isolates, randomly selected from 165 human group A rotavirus-positive samples, were G and P characterized by a reverse transcription (RT)-PCR assay using a seminested multiplex method. Rotaviruses of the G3 genotype were found to be the predominant strain (78%), followed by G2 (14%) and GI (2%). Mixed infections, as shown by combinations of G3 and G2 (4%) and G3 and G1 (2%), were also observed. P typing showed P[4] (72.34%) to be the prevalent strain, followed by P[6] (21.3%), P[8] (2.13%), and a combination of P [4] and P [6] (4.3%).

Item Type: Article
Keywords: Polymerase-chain-reaction, nucleic-acid, specimens, pcr, Child, Preschool, Gastroenteritis, epidemiology, virology, Genotype, Ghana, epidemiology, Human, Infant, Infant, Newborn, Reverse Transcriptase Polymerase Chain Reaction, Rotavirus, classification, genetics, Rotavirus Infections, epidemiology, virology, Rural Population, Support, Non-U.S. Gov't
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
PubMed ID: 11326029
Web of Science ID: 168527900056
URI: http://researchonline.lshtm.ac.uk/id/eprint/16910

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