Acute-phase HDL in phospholipid transfer protein (PLTP)- mediated HDL conversion


Pussinen, PJ; Malle, E; Metso, J; Sattler, W; Raynes, JG; Jauhiainen, M; (2001) Acute-phase HDL in phospholipid transfer protein (PLTP)- mediated HDL conversion. Atherosclerosis, 155 (2). pp. 297-305. ISSN 0021-9150 DOI: https://doi.org/10.1016/S0021-9150(00)00568-2

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Abstract

In reverse cholesterol transport, plasma phospholipid transfer protein (PLTP) converts high density lipoprotein, (HDL,) into two new subpopulations. HDL,-like particles and prep-HDL. During the acute-phase reaction (APR), serum amyloid A (SAA) becomes the predominant apolipoprotein on HDL. Displacement of apo A-I by SAA and subsequent remodeling of HDL during the APR impairs cholesterol efflux From peripheral tissues, and might thereby change substrate properties of HDL for lipid transfer proteins. Therefore, the aim of this work was to study the properties of SAA-containing HDL in PLTP-mediated conversion. Enrichment of HDL by SAA was performed in vitro and in vitro and the SAA content in HDL varied between 32 and 58 mass%. These HDLs were incubated with PLTP, and the conversion products were analyzed for their size, composition, mobility in agarose gels, and apo A-I degradation. Despite decreased apo A- I concentrations, PLTP facilitated the conversion of acute- phase HDL (AP-HDL) more effectively than the conversion of native HDL3, and large fusion particles with diameters of 10.5, 12.0, and 13.8 nm were generated. The ability of PLTP to release pre beta from,AP-HDL was more profound than from native HDL3. Pre beta -HDL formed contained fragmented apo A-I with a molecular mass of about 23 kDa. The present findings suggest that PLTP-mediated conversion of AP-HDL is not impaired, indicating that the production of pre beta -HDL is functional during the ARP. However, PLTP-mediated in vitro degradation of apo A-I in AP-HDL was more effective than that of native HDL, which may be associated with a faster catabolism of inflammatory HDL. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

Item Type: Article
Keywords: inflammation, acute-phase response, serum amyloid A, phospholipid transfer protein, HDL conversion, HDL subclasses, Pre beta-HDL, atherosclerosis, Serum amyloid-a, high-density-lipoprotein, cholesteryl ester, transfer, triglyceride-enrichment, particle fusion, apoa-i, lpa-i, plasma, apolipoprotein, saa, Acute-Phase Reaction, metabolism, Amyloid Protein SAA, metabolism, Animal, Carrier Proteins, metabolism, Cholesterol, metabolism, Comparative Study, Human, Lipoproteins, HDL, metabolism, Membrane Proteins, metabolism, Particle Size, Rabbits, Support, Non-U.S. Gov't
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
PubMed ID: 11254899
Web of Science ID: 167719800004
URI: http://researchonline.lshtm.ac.uk/id/eprint/16497

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