Tissue cytokine responses in canine visceral leishmaniasis

Quinnell, RJ; Courtenay, O; Shaw, MA; Day, MJ; Garcez, LM; Dye, C; Kaye, PM; (2001) Tissue cytokine responses in canine visceral leishmaniasis. The Journal of infectious diseases, 183 (9). pp. 1421-4. ISSN 0022-1899

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To elucidate the local tissue cytokine response of dogs infected with Leishmania chagasi, cytokine mRNA levels were measured in bone marrow aspirates from 27 naturally infected dogs from Brazil and were compared with those from 5 uninfected control animals. Interferon-gamma mRNA accumulation was enhanced in infected dogs and was positively correlated with humoral (IgG1) but not with lymphoproliferative responses to Leishmania antigen in infected dogs. Increased accumulation of mRNA for interleukin (IL)-4, IL-10, and IL-18 was not observed in infected dogs, and mRNA for these cytokines did not correlate with antibody or proliferative responses. However, infected dogs with detectable IL-4 mRNA had significantly more severe symptoms. IL-13 mRNA was not detectable in either control or infected dogs. These data suggest that clinical symptoms are not due to a deficiency in interferon-gamma production. However, in contrast to its role in human visceral leishmaniasis, IL-10 may not play a key immunosuppressive role in dogs.

Item Type: Article
Keywords: Gamma-interferon, kala-azar, interleukin-10, expression, infantum, dogs, Actins, Animal, Antibodies, Protozoan, blood, Biopsy, Needle, Bone Marrow, chemistry, immunology, Case-Control Studies, Cytokines, analysis, genetics, Disease Models, Animal, Dogs, Immunoglobulin G, blood, Interferon Type II, biosynthesis, genetics, Interleukin-10, analysis, genetics, Interleukin-13, analysis, genetics, Interleukin-18, analysis, genetics, Interleukin-4, analysis, genetics, Leishmania, immunology, Leishmaniasis, Visceral, immunology, RNA, Messenger, analysis, Reverse Transcriptase Polymerase Chain Reaction, Support, Non-U.S. Gov't
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Research Centre: Leishmaniasis Group
PubMed ID: 11294678
Web of Science ID: 167993400018
URI: http://researchonline.lshtm.ac.uk/id/eprint/16443


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