Mucosal and systemic immune responses to plasmid protein pgp3 in patients with genital and ocular Chlamydia trachomatis infection


Ghaem-Maghami, S; Ratti, G; Ghaem-Maghami, M; Comanducci, M; Hay, PE; Bailey, RL; Mabey, DCW; Whittle, HC; Ward, ME; Lewis, DJM; (2003) Mucosal and systemic immune responses to plasmid protein pgp3 in patients with genital and ocular Chlamydia trachomatis infection. Clinical and experimental immunology, 132 (3). pp. 436-442. ISSN 0009-9104 DOI: https://doi.org/10.1046/j.1365-2249.2003.02163.x

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Abstract

The circulating and cervical B cell responses to Chlamydia trachomatis plasmid protein pgp3 were characterized in children and adults with ocular or genital chlamydial infection using the enzyme-linked immunospot assay (ELISPOT) and ELISA. No pgp3-specific ASCs were detected in healthy controls, but predominantly IgA ASCs were detected in UK adults with uncomplicated cervicitis or urethritis (P = 0.03, 0.019). In patients with extragenital complications or pelvic inflammatory disease a mixed response with more IgG and IgM ASCs was evident, suggesting a breach of mucosal immune compartmentalization with more extensive infection. In women with chlamydial cervicitis, ASCs secreting predominantly IgA, but also IgG, to pgp3 were present in cervix at presentation, with a frequency 30-50 times higher than blood. Cervical ASC numbers, especially IgG, fell markedly six weeks after antibiotic treatment. We detected principally IgA pgp3-specific antibody secreting cells (ASCs) in children resident in a Gambian endemic area, with a trend towards suppression of IgA responses during intense trachomatous inflammation (P = 0.06), as previously reported for other chlamydial antigens, and in keeping with the findings in genital disease. These data provide a rationale for further studies of immune responses to pgp3 in humans and animal models of chlamydia-induced disease, and its potential use in diagnostic assays and protective immunization strategies.

Item Type: Article
Keywords: chlamydia, B cell, ELISPOT, pgp3, trachoma, antibody-secreting cells, diagnostic-value, antigens, immunoglobulin, expression, enumeration, vaccination, serology, humans, gene, Adult, Antibiotics, therapeutic use, Antibodies, Bacterial, biosynthesis, Antibody Specificity, Antibody-Producing Cells, immunology, Antigens, Bacterial, immunology, Bacterial Proteins, immunology, Cervicitis, drug therapy, immunology, microbiology, Child, Chlamydia Infections, drug therapy, immunology, Chlamydia trachomatis, immunology, Enzyme-Linked Immunosorbent Assay, methods, Female, Human, Immunity, Mucosal, Immunoglobulin A, biosynthesis, Immunoglobulin G, biosynthesis, Immunoglobulin M, biosynthesis, Male, Support, Non-U.S. Gov't, Trachoma, immunology, microbiology, Urethritis, immunology, microbiology
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
Research Centre: Malaria Centre
PubMed ID: 12780690
Web of Science ID: 183693900009
URI: http://researchonline.lshtm.ac.uk/id/eprint/16081

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