Characterisation of Leishmania donovani promastigotes resistant to hexadecylphosphocholine (miltefosine)


Seifert, K; Matu, S; Javier Perez-Victoria, F; Castanys, S; Gamarro, F; Croft, SL; (2003) Characterisation of Leishmania donovani promastigotes resistant to hexadecylphosphocholine (miltefosine). International journal of antimicrobial agents, 22 (4). pp. 380-387. ISSN 0924-8579 DOI: 10.1016/s0924-8579(03)00125-0

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Abstract

Leishmania donovani promastigote lines resistant to hexadecylphosphocholine (HePC, miltefosine) at 2.5, 5.0, 10.0, 20.0 and 40.0 microM were developed in vitro by continuous step-wise drug pressure. The 40 microM line was 15 times more resistant to HePC than the wild-type clone and showed cross-resistance to the ether lipid ET-18-OCH(3) (edelfosine) but not to the standard anti-leishmanial drugs. Resistance was stable up to 12 weeks in drug-free culture medium. No amplification of specific genes, including the multidrug resistance P-glycoprotein gene, could be detected in the resistant parasites.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Research Centre: Antimicrobial Resistance Centre (AMR)
Leishmaniasis Group
Malaria Centre
Neglected Tropical Diseases Network
PubMed ID: 14522101
Web of Science ID: 186084900004
URI: http://researchonline.lshtm.ac.uk/id/eprint/15836

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