Contrasting roles for IL-10 in protective immunity to different life cycle stages of intestinal nematode parasites


Helmby, H; Grencis, RK; (2003) Contrasting roles for IL-10 in protective immunity to different life cycle stages of intestinal nematode parasites. European journal of immunology, 33 (9). pp. 2382-90. ISSN 0014-2980 DOI: 10.1002/eji.200324082

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Abstract

Expulsion of the gastro-intestinal nematode Trichinella spiralis is associated with a pronounced mastocytosis mediated by a T helper (Th) 2 type response involving interleukin (IL)-4 and IL-13. Here we demonstrate that IL-10 is a key regulator of protective immune responses against T. spiralis in vivo. IL-10 knockout mice or normal mice treated with a neutralizing anti-IL-10 receptor antibody are highly susceptible to a primary T. spiralis infection and show significantly delayed adult worm expulsion. Depletion of IL-10 resulted in elevated Th1 and Th2 cytokine responses but significantly reduced numbers of mucosal mast cells in the jejunum. Interestingly, the increase in IFN-gamma detected in the absence of IL-10 resulted in increased immunity to larval stages. Hence, IL-10 has a negative effect on immunity to the tissue dwelling larval stages of T. spiralis but plays a significant biological role as an in vivo regulator of intestinal mast cell responses and is crucially involved in protection against adult stages of intestinal parasites in vivo.

Item Type: Article
Keywords: Animal, Cytokines/metabolism, Interleukin-10/genetics/*metabolism, Intestinal Diseases, Parasitic/*immunology, Larva/immunology/metabolism, Lymph Nodes/immunology/metabolism, Mast Cells/immunology, Mesentery/immunology/metabolism, Mice, Mice, Knockout, Mucous Membrane/immunology, Muscle, Skeletal/metabolism, Nematode Infections/*immunology, Support, Non-U.S. Gov't, Trichinella spiralis/immunology/metabolism, Animal, Cytokines, metabolism, Interleukin-10, genetics, metabolism, Intestinal Diseases, Parasitic, immunology, Larva, immunology, metabolism, Lymph Nodes, immunology, metabolism, Mast Cells, immunology, Mesentery, immunology, metabolism, Mice, Mice, Knockout, Mucous Membrane, immunology, Muscle, Skeletal, metabolism, Nematode Infections, immunology, Support, Non-U.S. Gov't, Trichinella spiralis, immunology, metabolism
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Research Centre: Neglected Tropical Diseases Network
PubMed ID: 12938214
Web of Science ID: 185323200004
URI: http://researchonline.lshtm.ac.uk/id/eprint/15636

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