Protection against plague afforded by immunisation with DNA vaccines optimised for expression of the Yersinia pestis V antigen


Garmory, HS; Freeman, D; Brown, KA; Titball, RW; (2004) Protection against plague afforded by immunisation with DNA vaccines optimised for expression of the Yersinia pestis V antigen. Vaccine, 22 (8). pp. 947-957. ISSN 0264-410X DOI: 10.1016/j.vaccine.2003.09.020

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Abstract

DNA vaccine vectors were produced which were optimised for expression of the Yersinia pestis V antigen in the BALB/c mouse model. Six different eukaryotic promoters were compared, resulting in the selection of the CMV promoter with an additional translational enhancer downstream. Surprisingly, alteration of the codon usage of the lcrV gene encoding V antigen for expression in murine cells was not found to improve the antibody responses generated against V antigen. The DNA vaccine was subsequently evaluated in its delivery via intramuscular injection compared to gene-gun administration. Gene-gun delivery was found to induce significantly higher V antigen-specific antibody responses and also afforded the highest level of protection against E pestis challenge. In addition, the protection achieved could be increased by using a 'prime and boost' strategy, administering the DNA vaccine followed by recombinant V antigen. These results show promise for a DNA vaccine against plague. Crown Copyright (C) 2003 Published by Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: plague, V antigen, Yersinia pestis, DNA vaccine, Codon usage, gene-gun, immune-responses, intramuscular, inoculation, plasmodium-falciparum, active immunization, pneumonic plague, subunit vaccine, protein, virus
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Vaccine Centre
PubMed ID: 15161071
Web of Science ID: 189266800005
URI: http://researchonline.lshtm.ac.uk/id/eprint/14908

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