Protection afforded by heat shock protein 60 from Francisella tularensis is due to copurified lipopolysaccharide


Hartley, MG; Green, M; Choules, G; Rogers, D; Rees, DGC; Newstead, S; Sjostedt, A; Titball, RW; (2004) Protection afforded by heat shock protein 60 from Francisella tularensis is due to copurified lipopolysaccharide. Infection and immunity, 72 (7). p. 4109. ISSN 0019-9567 DOI: 10.1128/IAI.72.7.4109-4113.2004

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Abstract

Heat shock proteins (Hsps) have attracted significant attention as protective antigens against a range of diseases caused by bacterial pathogens. However, more recently there have been suggestions that the protective response is due to the presence of peptide components other than Hsps. We have shown that mice that had been immunized with purified heat shock protein 60 (Hsp60) isolated from Francisella tularensis were protected against a subsequent challenge with some strains of the bacterium. However, this protection appeared to be due to trace amounts of lipopolysaccharide, which were too low to be detected by using the Limulus amoebocyte lysate assay. This finding raises the possibility that the protection afforded by other bacterial Hsp60 proteins may be due to trace quantities of polysaccharide antigens carried by and acting in conjunction with the Hsps.

Item Type: Article
Keywords: Heat-shock-protein, mycobacterium-tuberculosis, immune-, response, t-cells, balb/c mice, vaccine, hsp60, lvs, immunization, infection
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 15213156
Web of Science ID: 222282800048
URI: http://researchonline.lshtm.ac.uk/id/eprint/14562

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