Novel azasterols as potential agents for treatment of leishmaniasis and trypanosomiasis


Lorente, SO; Rodrigues, JCF; Jimenez, CJ; Joyce-Menekse, M; Rodrigues, C; Croft, SL; Yardley, V; de Luca-Fradley, K; Ruiz-Perez, LM; Urbina, J; de Souza, W; Pacanowska, DG; Gilbert, IH; (2004) Novel azasterols as potential agents for treatment of leishmaniasis and trypanosomiasis. Antimicrobial agents and chemotherapy, 48 (8). p. 2937. ISSN 0066-4804 DOI: https://doi.org/10.1128/AAC.48.8.2937-2950.2004

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Abstract

This paper describes the design and evaluation of novel azasterols as potential compounds for the treatment of leishmaniasis and other diseases caused by trypanosomatid parasites. Azasterols are a known class of (S)-adenosyl-L- methionine: Delta(24)-sterol methltransferase(24-SMT) inhibitors in fungi, plants, and some parasitic protozoa. The compounds prepared showed activity at micromolar and nanomolar concentrations when tested against Leishmania spp. and Trypanosoma spp. The enzymatic and sterol composition studies indicated that the most active compounds acted by inhibiting 24-SMT. The role of the free hydroxyl group at position 3 of the sterol nucleus was also probed. When an acetate was attached to the 3beta-OH, the compounds did not inhibit the enzyme but had an effect on parasite growth and the levels of sterols in the parasite, suggesting that the acetate group was removed in the organism. Thus, an acetate group on the 3beta-OH may have application as a prodrug. However, there may be an additional mode(s) of action for these acetate derivatives. These compounds were shown to have ultrastructural effects on Leishmania amazonensis promastigote membranes, including the plasma membrane, the mitochondrial membrane, and the endoplasmic reticulum. The compounds were also found to be active against the bloodstream form (trypomastigotes) of Trypanosoma brucei rhodesiense, a causative agent of African trypanosomiasis.

Item Type: Article
Keywords: Methyl transferase inhibitors, schizotrypanum cruzi, in-vitro, delta-24-sterol methyltransferase, ultrastructural alterations, sterol composition, saccharomyces-cerevisiae, ergosterol, biosynthesis, candida-albicans, brucei
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Research Centre: Leishmaniasis Group
Neglected Tropical Diseases Network
PubMed ID: 15273104
Web of Science ID: 222998300023
URI: http://researchonline.lshtm.ac.uk/id/eprint/14435

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