Measuring lymphocyte kinetics in tropical field settings


Ghattas, H; Darboe, BM; Wallace, DL; Griffin, GE; Prentice, AM; MacAllan, DC; (2005) Measuring lymphocyte kinetics in tropical field settings. Transactions of the Royal Society of Tropical Medicine and Hygiene, 99 (9). pp. 675-685. ISSN 0035-9203 DOI: 10.1016/j.trstmh.2005.02.005

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Abstract

Studies involving in-vivo labelling of lymphocyte DNA with 6,6-(2)H2-glucose to track T-cell turnover have contributed to understanding lymphocyte homeostasis in health and disease. Applying such studies in tropical settings (where diseases that affect T-cells are prevalent) requires protocol modifications including non-intravenous label administration, applicability in outpatient facilities, and T-cell sorting methods independent of a fluorescence activated cell sorter (FACS). Such protocols were validated in UK pilot studies and applied in The Gambia. Healthy adult subjects (n = 12) were recruited from three Gambian villages. 6,6-H-2(2)-glucose was administered orally in an outpatient clinic and T-cell subpopulations isolated from peripheral blood using plastic adherence, and Multisort (TM) magnetic cell sorting (MACS (TM)) to obtain CD8 + CD45RO+, CD8-CD45RO+, CD8 + CD45RO- and CD8-CD45RO- subsets. To achieve high cell purity and yield, CD45RO- cells were reincubated with CD45R0 beads. T-cell proliferation and disappearance were quantified using gas chromatography mass spectrometry. Results were consistent with those of other studies showing higher turnover in memory (CD45RO+) than in naive (CD45RO-) T-cell subsets, and an association between recent cell proliferation and susceptibility to cell death. Cell kinetics research is applicable in tropical settings, and can contribute to further understanding the regulation of adaptive immunity in response to infections and other insults. (c) 2005 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: T-lymphocyte, kinetics, stable isotope labelling, cell sorting, MACS, The Gambia, T-cell kinetics, reference ranges, rapid turnover, in-vivo, dna, humans, blood, proliferation, ethiopians
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Population Health (2012- ) > Dept of Nutrition and Public Health Interventions Research (2003-2012)
Faculty of Epidemiology and Population Health > Dept of Population Health (2012- )
Research Centre: Centre for Global Non-Communicable Diseases (NCDs)
PubMed ID: 15985275
Web of Science ID: 230881700006
URI: http://researchonline.lshtm.ac.uk/id/eprint/13251

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