Programmed Transcription of the var Gene Family, but not of stevor, in Plasmodium falciparum Gametocytes.


Sharp, S; Lavstsen, T; Fivelman, QL; Saeed, M; McRobert, L; Templeton, TJ; Jensen, AT; Baker, DA; Theander, TG; Sutherland, CJ; (2006) Programmed Transcription of the var Gene Family, but not of stevor, in Plasmodium falciparum Gametocytes. Eukaryotic cell, 5 (8). pp. 1206-14. ISSN 1535-9778 DOI: https://doi.org/10.1128/EC.00029-06

Full text not available from this repository. (Request a copy)

Abstract

The var genes encode Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) proteins, a set of highly diverse surface-expressed proteins that mediate adhesion of erythrocytes infected with asexual blood-stage parasites to host endothelium. Switching among expressed PfEMP1 variants in the course of a blood-stage infection is a key component of antigenic variation, and thus immune evasion, by the parasite. The majority of var loci are found in the subtelomeric regions of P. falciparum chromosomes associated with members of other multigene families, including stevor. Both PfEMP1 and STEVOR are expressed in gametocytes, the transmissible parasite stage, but the role of these proteins in the biology of sexual-stage parasites remains unknown. PfEMP1 may continue to mediate antigenic variation in gametocytes, which need to persist in the host for many days before reaching maturity. Using quantitative reverse transcription-PCR and Northern hybridization, we demonstrate that transcription of a defined subset of type C var loci occurs during gametocyte development in vitro. This transcriptional program occurs in gametocytes regardless of the var expression phenotype of their asexual progenitors and therefore is subject to regulatory processes distinct from those that manage antigenic variation in the asexual parasite. In contrast, the same stevor variants are transcribed in both gametocytes and their asexual progenitors. We also provide evidence that for both asexual parasites and gametocytes, var and stevor transcription patterns are not linked to each other.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Research Centre: Malaria Centre
PubMed ID: 16896206
Web of Science ID: 239778700004
URI: http://researchonline.lshtm.ac.uk/id/eprint/11654

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
295Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item