Assessment of rDNA IGS as a molecular marker in the Simulium damnosum complex


Morales-Hojas, R; Post, RJ; Cheke, RA; Wilson, MD; (2002) Assessment of rDNA IGS as a molecular marker in the Simulium damnosum complex. Medical and veterinary entomology, 16 (4). pp. 395-403. ISSN 0269-283X DOI: https://doi.org/10.1046/j.1365-2915.2002.00391.x

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Abstract

For five cytospecies of the Simulium damnosum Theobald complex of blackflies (Diptera: Simuliidae) from West Africa, both ends of the intergenic spacer region (IGS) of the rDNA have been sequenced with the aim of developing specific molecular markers. No specific differences in these two regions were detected between Simulium sanctipauli V. & D., Simulium sirbanum V. & D., Simulium soubrense V. & D., Simulium squamosum Enderlein and Simulium yahense V. & D., except in the number of A subrepeats at the 5' end of the IGS (two in S. squamosum and four or five in the others) and in position 310 of the 3' end (a C in S. squamosum and a G in the others). However, genetic distances within and between species overlapped. These DNA sequences had no strong phylogenetic signal, and the trees obtained were mostly unresolved. Although most sequences from S. squamosum clustered together, a few of them were more similar to those in other cytospecies. These results could be explained either by hybridization with genetic introgression or by ancestral polymorphism and recent speciation.

Item Type: Article
Keywords: Africa, Western, Animals, DNA, Ribosomal Spacer, Evolution, Molecular, Genes, Insect, Genetic Markers, Molecular Sequence Data, Phylogeny, Research Support, Non-U.S. Gov't, Simuliidae, Species Specificity, Variation (Genetics), Africa, Western, Animals, DNA, Ribosomal Spacer, genetics, Evolution, Molecular, Genes, Insect, Genetic Markers, genetics, Molecular Sequence Data, Phylogeny, Research Support, Non-U.S. Gov't, Simuliidae, classification, genetics, Species Specificity, Variation (Genetics)
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Disease Control
PubMed ID: 12510892
Web of Science ID: 179803600008
URI: http://researchonline.lshtm.ac.uk/id/eprint/11065

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