Transcriptional adaptation of pneumococci and human pharyngeal cells in the presence of a virus infection.


Kimaro Mlacha, SZ; Peret, TC; Umar, N; Romero-Steiner, S; Dunning Hotopp, JC; Ishmael, N; Grinblat-Huse, V; Riley, DR; Erdman, DD; Carlone, GM; Sampson, J; Scott, JA; Tettelin, H; (2013) Transcriptional adaptation of pneumococci and human pharyngeal cells in the presence of a virus infection. BMC Genomics, 14. p. 378. ISSN 1471-2164 DOI: 10.1186/1471-2164-14-378

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Abstract

BACKGROUND Viral upper respiratory tract infections are associated with increased colonization by Streptococcus pneumoniae but the mechanisms underlying this relationship are unclear. The objective of this study is to describe a comprehensive picture of the cellular interaction between the adhering bacteria and host cells in the presence or absence of a viral co-infection. RESULTS Gene expression profiles of Detroit-562 pharyngeal cells, which were either mock-infected or infected with human respiratory syncytial virus (RSV) or human parainfluenza virus 3 (HPIV3), were analyzed using human microarrays. Transcription response of S. pneumoniae strain TIGR4 (serotype 4) in the presence of either mock- or viral-infected cells was analyzed by pneumococcal microarray. Significantly regulated genes were identified by both significance analysis of microarray (SAM) and a ≥ 2-fold change ratio cut-off. The adherence of S. pneumoniae to human pharyngeal cells was significantly augmented in the presence of RSV or HPIV3 infection. Global gene expression profiling of the host cells during infection with RSV or HPIV3 revealed increased transcription of carcinoembryonic antigen-related cell adhesion molecules (CEACAM1), CD47, fibronectin, interferon-stimulated genes and many other host cell adhesion molecules. Pneumococci increased transcription of several genes involved in adhesive functions (psaA, pilus islet), choline uptake and incorporation (lic operon), as well as transport and binding. CONCLUSIONS We have identified a core transcriptome that represents the basic machinery required for adherence of pneumococci to D562 cells infected or not infected with a virus. These bacterial genes and cell adhesion molecules can potentially be used to control pneumococcal adherence occurring secondary to a viral infection.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Disease Control
Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
PubMed ID: 23742656
Web of Science ID: 320325100001
URI: http://researchonline.lshtm.ac.uk/id/eprint/1105535

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