Defining the origin of Plasmodium falciparum resistant dhfr isolates in Senegal


Ndiaye, D; Daily, JP; Sarr, O; Ndir, O; Gaye, O; Mboup, S; Roper, C; Wirth, DF; (2006) Defining the origin of Plasmodium falciparum resistant dhfr isolates in Senegal. Acta tropica, 99 (1). pp. 106-111. ISSN 0001-706X DOI: 10.1016/j.actatropica.2006.07.002

Full text not available from this repository.

Abstract

We previously reported a high baseline prevalence of mutations in the dhfr and dhps genes of Plasmodium falciparum throughout Senegal. The highest prevalence of the triple dhfr pyrimethamine associated mutations were found in isolates obtained in the western part of the country near the capital city of Dakar. In this study, we sought out to determine the relatedness of dhfr wild type and mutated strains by analyzing three microsatellite regions upstream of the dhfr locus. Twenty-six of the 31 wild type strains had a unique microsatellite pattern. In contrast, of the 17 isolates containing the triple mutation in dhfr, 11 had an identical microsatellite pattern. Diverse geographical isolates in Senegal containing the triple dhfr mutation have arisen from a limited number of ancestral strains. In addition, we demonstrate that these isolates have shared ancestry with the previously reported triple mutation haplotype found in Tanzania, South Africa, and southeast Asia. This common ancestry may have implications for the malaria control strategy for reducing the spread of sulfadoxine-pyrimethamine resistance in Senegal and elsewhere in Africa. (c) 2006 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: Plasmodium falciparum, drug resistance, Senegal, dhfr, sulfadoxine-pyrimethamine, MALARIA PARASITES, DRUG-RESISTANCE, MUTATIONS, ALLELES
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Antimicrobial Resistance Centre (AMR)
Malaria Centre
PubMed ID: 16905111
Web of Science ID: 241227000017
URI: http://researchonline.lshtm.ac.uk/id/eprint/10541

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
290Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item