Vital functions of the malarial ookinete protein, CTRP, reside in the A domains

Ramakrishnan, C; Dessens, JT; Armson, R; Pinto, SB; Talman, AM; Blagborough, AM; Sinden, RE; (2011) Vital functions of the malarial ookinete protein, CTRP, reside in the A domains. International journal for parasitology, 41 (10). pp. 1029-1039. ISSN 0020-7519 DOI:

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The transformation of malaria ookinetes into oocysts occurs in the mosquito midgut and is a major bottleneck for parasite transmission. The secreted ookinete surface protein, circumsporozoite- and thrombospondin-related adhesive protein (TRAP)-related protein (CTRP), is essential for this transition and hence constitutes a potential target for malaria transmission blockade. CTRP is a modular multidomain protein containing six tandem von Willebrand factor A-like (A) domains and seven tandem thrombospondin type I repeat-like (TS) domains. Here we present, to our knowledge, the first structure-function analysis of CTRP using genetically modified Plasmodium berghei parasites expressing mutant versions of the ctrp gene. Our data show that the A domains of CTRP are critical for ookinete gliding motility and oocyst formation whilst, unexpectedly, its TS domains are fully redundant. These results may have important implications for the design of CTRP-based transmission blocking strategies. (C) 2011 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: CTRP, Transmission, Motility, Invasion, Plasmodium berghei, Anopheles, host-cell invasion, thrombospondin-related protein, high-efficiency, transfection, mosquito anopheles-gambiae, trap-like protein, plasmodium-berghei, apicomplexan parasites, molecular characterization, anonymous protein, gliding motility, ater ca, 1991, v112, p1031
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Malaria Centre
PubMed ID: 21729699
Web of Science ID: 294519200003


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